This paper presents the results achieved throughout the process of preparing the ground to develop a collaboratory in an Engineering R&D organization. This case study is part of a broader research project engaged in building a collaboratory in order to share knowledge and resources among the Portuguese State laboratories. In the process of preparing the ground to develop the collaboratory in the first of the laboratories studied, an information audit was conducted and an online survey was launched. The survey targeted 241 people, including mainly professional researchers, but also research trainees and some technical staff integrating the research teams. The questionnaire was designed so as to collect data on the organization’s information management and information culture, and on the information flows taking place, and their relationship with the objectives of the organization. The questionnaire comprised two distinct and independent parts. The first (on the organization’s information culture and information management) obtained seventy nine responses, while the second (information flows) achieved ninety two, corresponding to 32,8% and 38,2% of the total population, respectively. The work carried out provided the basic requirements for the task of developing a software infrastructure to support the collaboratory, addressing the various aspects of collaborative tools, information archiving, hierarchical tag classification, search, transparent integration of the user local environment with the platform and remote control of scientific instruments.