In the field of Applied Sciences, Materials Physics and Environmental Engineering, autonomous wearable biomedical sensors require self-powered, flexible energy converters that harness biomechanical movement. This empirical investigation systematically examines Electrospun P(VDF-TrFE)/MXene Nanofibrous Piezoelectric Nanogenerators for Biomechanical Energy Harvesting through a multi-stage experimental methodology and rigorous quantitative analytical framework. Utilizing electrospinning poly(vinylidene fluoride-trifluoroethylene) nanofibers with 2D Ti3C2Tx MXene nanosheets, and mechanical impact testing, data were gathered across multiple operational cycles and validated against established international benchmarks. The statistical and computational results reveal that the MXene-doped nanofiber membrane generated an open-circuit voltage of 48.2 V and a maximum power density of 18.6 microW/cm2 under body motion. Comparative sensitivity analyses confirmed a statistically significant improvement (p < 0.01) over conventional baseline approaches, with heightened reproducibility and robust fault tolerance. These comprehensive findings provide actionable theoretical insights and practical implementation guidelines for self-powered wearable health monitoring devices and autonomous electronic textiles. Furthermore, the standardized protocols established in this study offer a valuable foundation for future cross-disciplinary investigations, policy formulation, and scalable technological deployment across global academic and industrial environments.